##gff-version  3
# Sequence Data: seqnum=1;seqlen=36347;seqhdr="bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction Generated by Gibson/Homology Cloning (bb - bb_AKM_TRP1, NZ_VNWI01000017 amplicon 1 (Rfn) Yeast assembly, NZ_VNWI01000023 amplicon 2 (Mod), NZ_VNWI01000032 amplicon 3.1 (Main Nif cluster) PCR Product, NZ_VNWI01000032 amplicon 3.2 (Main Nif cluster) PCR Product)"
# Model Data: version=Prodigal.v2.6.3;run_type=Metagenomic;model="25|Marinobacter_aquaeolei_VT8|B|57.3|11|1";gc_cont=57.30;transl_table=11;uses_sd=1
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	152	415	28.3	+	0	ID=1_1;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.443;conf=99.85;score=28.25;cscore=27.46;sscore=0.79;rscore=1.44;uscore=-4.48;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	495	935	48.1	+	0	ID=1_2;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.556;conf=100.00;score=48.08;cscore=34.85;sscore=13.23;rscore=8.92;uscore=0.48;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	1013	1594	77.7	+	0	ID=1_3;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.541;conf=100.00;score=77.67;cscore=71.61;sscore=6.06;rscore=1.44;uscore=0.79;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	1594	2172	41.7	+	0	ID=1_4;partial=00;start_type=ATG;rbs_motif=None;rbs_spacer=None;gc_cont=0.596;conf=100.00;score=49.39;cscore=44.28;sscore=5.12;rscore=-6.26;uscore=-0.17;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	2165	4351	250.0	+	0	ID=1_5;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.636;conf=99.99;score=251.30;cscore=244.11;sscore=7.18;rscore=1.44;uscore=0.67;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	4352	5404	122.9	+	0	ID=1_6;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.592;conf=100.00;score=124.15;cscore=111.64;sscore=12.51;rscore=8.92;uscore=-1.51;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	5415	6035	78.8	+	0	ID=1_7;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.572;conf=100.00;score=79.98;cscore=67.89;sscore=12.09;rscore=8.92;uscore=-1.82;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	6039	6746	94.3	+	0	ID=1_8;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.562;conf=100.00;score=95.52;cscore=81.52;sscore=14.00;rscore=8.92;uscore=0.01;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	6739	7374	59.4	+	0	ID=1_9;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.549;conf=100.00;score=60.62;cscore=57.37;sscore=3.25;rscore=1.44;uscore=-3.26;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	7551	8609	136.2	-	0	ID=1_10;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.612;conf=100.00;score=137.41;cscore=132.59;sscore=4.81;rscore=1.44;uscore=-1.71;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	8612	9301	74.7	-	0	ID=1_11;partial=00;start_type=ATG;rbs_motif=3Base/5BMM;rbs_spacer=13-15bp;gc_cont=0.600;conf=100.00;score=80.08;cscore=74.49;sscore=5.60;rscore=-4.13;uscore=0.48;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	9301	10074	97.9	-	0	ID=1_12;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.571;conf=100.00;score=97.86;cscore=82.50;sscore=15.35;rscore=8.92;uscore=2.60;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	10220	10369	12.8	-	0	ID=1_13;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.507;conf=94.26;score=12.17;cscore=3.56;sscore=8.61;rscore=5.24;uscore=1.76;tscore=2.25;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	10523	11311	113.3	+	0	ID=1_14;partial=00;start_type=ATG;rbs_motif=AGGA;rbs_spacer=5-10bp;gc_cont=0.598;conf=100.00;score=113.29;cscore=100.23;sscore=13.06;rscore=8.62;uscore=0.61;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	11379	12848	203.8	+	0	ID=1_15;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.598;conf=99.99;score=203.79;cscore=196.06;sscore=7.73;rscore=1.44;uscore=2.47;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	13012	16527	492.6	-	0	ID=1_16;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.614;conf=99.99;score=491.99;cscore=480.25;sscore=11.74;rscore=8.92;uscore=-0.36;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	16932	17813	164.0	+	0	ID=1_17;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.588;conf=100.00;score=163.96;cscore=142.79;sscore=21.17;rscore=14.12;uscore=3.21;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	17827	19278	235.7	+	0	ID=1_18;partial=00;start_type=ATG;rbs_motif=AGGA;rbs_spacer=5-10bp;gc_cont=0.596;conf=99.99;score=236.79;cscore=224.92;sscore=11.86;rscore=8.62;uscore=-1.72;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	19334	20896	249.7	+	0	ID=1_19;partial=00;start_type=ATG;rbs_motif=GGAGG;rbs_spacer=5-10bp;gc_cont=0.582;conf=99.99;score=250.41;cscore=235.45;sscore=14.96;rscore=10.97;uscore=-0.52;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	20936	21154	35.6	+	0	ID=1_20;partial=00;start_type=ATG;rbs_motif=AGGAGG;rbs_spacer=3-4bp;gc_cont=0.630;conf=99.98;score=36.48;cscore=21.18;sscore=15.29;rscore=13.49;uscore=-2.35;tscore=3.31;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	21165	21827	87.4	+	0	ID=1_21;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.661;conf=100.00;score=88.54;cscore=73.61;sscore=14.93;rscore=14.12;uscore=-4.19;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	22149	23522	205.3	+	0	ID=1_22;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.635;conf=99.99;score=204.67;cscore=191.62;sscore=13.05;rscore=8.92;uscore=0.95;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	23533	24918	155.9	+	0	ID=1_23;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.637;conf=100.00;score=157.10;cscore=138.91;sscore=18.19;rscore=14.12;uscore=-0.93;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	24905	25375	73.9	+	0	ID=1_24;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.609;conf=100.00;score=75.11;cscore=54.80;sscore=20.31;rscore=14.12;uscore=1.17;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	25570	26394	129.5	+	0	ID=1_25;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.619;conf=100.00;score=128.83;cscore=118.18;sscore=10.65;rscore=8.92;uscore=-1.44;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	26425	27627	157.5	+	0	ID=1_26;partial=00;start_type=ATG;rbs_motif=AGxAGG/AGGxGG;rbs_spacer=5-10bp;gc_cont=0.648;conf=100.00;score=158.49;cscore=152.49;sscore=6.00;rscore=3.18;uscore=-1.96;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	27642	28784	183.0	+	0	ID=1_27;partial=00;start_type=ATG;rbs_motif=GGAGG;rbs_spacer=5-10bp;gc_cont=0.668;conf=99.99;score=184.17;cscore=167.86;sscore=16.31;rscore=10.97;uscore=0.39;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	28784	29044	37.1	+	0	ID=1_28;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.598;conf=99.99;score=39.25;cscore=25.22;sscore=14.03;rscore=8.92;uscore=-0.83;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	29041	29487	61.7	+	0	ID=1_29;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.626;conf=100.00;score=63.00;cscore=43.27;sscore=19.73;rscore=14.12;uscore=0.48;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	29484	30284	94.5	+	0	ID=1_30;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.662;conf=100.00;score=96.21;cscore=76.96;sscore=19.25;rscore=14.12;uscore=-0.46;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	30307	30837	93.5	-	0	ID=1_31;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.606;conf=100.00;score=92.83;cscore=76.60;sscore=16.24;rscore=14.12;uscore=-1.07;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	31159	32646	217.9	+	0	ID=1_32;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.630;conf=99.99;score=217.89;cscore=198.69;sscore=19.19;rscore=14.12;uscore=1.24;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	32643	34217	237.2	+	0	ID=1_33;partial=00;start_type=ATG;rbs_motif=GGAGG;rbs_spacer=5-10bp;gc_cont=0.625;conf=99.99;score=238.52;cscore=222.01;sscore=16.51;rscore=10.97;uscore=0.41;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	34382	35788	146.4	+	0	ID=1_34;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.645;conf=100.00;score=146.43;cscore=140.26;sscore=6.17;rscore=1.44;uscore=0.90;tscore=3.83;
bb_-_bb_AKM_TRP1_-_NZ_VNWI01000017_amplicon_1_(Rfn)_Yeast_assembly_-_NZ_VNWI01000023_amplicon_2_(Mod)_(+2)_extraction	Prodigal_v2.6.3	CDS	35788	36291	56.4	+	0	ID=1_35;partial=00;start_type=ATG;rbs_motif=AGxAGG/AGGxGG;rbs_spacer=5-10bp;gc_cont=0.653;conf=100.00;score=60.79;cscore=52.50;sscore=8.30;rscore=3.18;uscore=-3.11;tscore=3.83;